chloroform extraction---become dark yellow
Posted 06 May 2010 - 12:06 PM
Posted 06 May 2010 - 12:42 PM
normally the phenol can turn yellow if old and oxidised...it's not recommended to use it then...
Today when I did phenol/chloroform extraction for the ChIP DNA, I found something strange. After adding chloroform to the aquar phase which extracted from phenol/chloroform/Isoamylalcohol, the organic phase turned to dark yellow...I found maybe due to chloroform is old, because the chloroform I used was a bit yellow...phenol/chlo/Isoamyl should be ok, it was a commercial one, the color is still light yellow...Is there anyone knows what is the dark yellow thing, will it break the DNA???
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.
Posted 06 May 2010 - 02:07 PM