Today when I did phenol/chloroform extraction for the ChIP DNA, I found something strange. After adding chloroform to the aquar phase which extracted from phenol/chloroform/Isoamylalcohol, the organic phase turned to dark yellow...I found maybe due to chloroform is old, because the chloroform I used was a bit yellow...phenol/chlo/Isoamyl should be ok, it was a commercial one, the color is still light yellow...Is there anyone knows what is the dark yellow thing, will it break the DNA???
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#2
hobglobin
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Posted 06 May 2010 - 12:42 PM
jenny0827, on May 6 2010, 10:06 PM, said:
Today when I did phenol/chloroform extraction for the ChIP DNA, I found something strange. After adding chloroform to the aquar phase which extracted from phenol/chloroform/Isoamylalcohol, the organic phase turned to dark yellow...I found maybe due to chloroform is old, because the chloroform I used was a bit yellow...phenol/chlo/Isoamyl should be ok, it was a commercial one, the color is still light yellow...Is there anyone knows what is the dark yellow thing, will it break the DNA???
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pDNA
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Posted 06 May 2010 - 02:07 PM
Oxidation of the phenol produces a pink/brown compound, and this compound will cause nicking of your DNA and degradation of your RNA.
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