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BstUI digestion


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#1 epicrazy

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Posted 06 May 2010 - 08:01 AM

I have ordered BstUI from Fermentas and they recommend 37 for 100% activity. But NEB mentions 20% activity at 37 and recommend 60C. Can someone please tell me if BstUI from Fermentas works at 37 or 60 and the number of hours of incubation for 1ug of DNA? Thanks! :D

#2 zhongmindai

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Posted 16 May 2010 - 12:24 AM

The BstUI from Fermentas is actually Bsh1236I (BstUI), it means Bsh1236I (Fermentas) and BstUI (NEB)recognize and cut the same sequences, but the the two enzymes are from different strains of Bacillus, so they fever different temperature conditions. 37 oC is recommended for Bsh1236I (BstUI).

Edited by zhongmindai, 16 May 2010 - 12:26 AM.

Zhong-Min Dai




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