1 reply to this topic

[rick112]

Hi

There are many protocols and methods with 230 nm as the wavelength for measurement of protein. So what contributes to absorbance at 230 nm in protein ???
Do any one has any article justifying the use of 230 nm for protein measurement ???

Thanks a lot

[Prep!]

rick112, on May 6 2010, 05:08 PM, said:

Hi

There are many protocols and methods with 230 nm as the wavelength for measurement of protein. So what contributes to absorbance at 230 nm in protein ???
Do any one has any article justifying the use of 230 nm for protein measurement ???

Thanks a lot

hey rick as far as i know at the wavelength 235 nm protein secondary and tertiary structures  are known to absorb light. I have attached a paper too.. hope it helps!!

Good luck!

Attached Files

•  235_nm_absorbance_of_protein.pdf   294.83K   582 downloads