CaCl2 for making competent cells
Posted 05 May 2010 - 07:53 PM
Posted 10 May 2010 - 06:48 AM
you can autoclave your glycerol as well, but I never did.
I use invitrogen's protocol for making TOP10
Posted 10 May 2010 - 06:50 AM
Posted 10 May 2010 - 05:13 PM
biochemical grade would be sufficient i guess. I normally sterile filter my solutions. Good luck!
Thank you Curits and Biolab12 for the answers, making me more confident
And Yes, we will surely sterile filter the solutions..