, and i'm going to adopt the protocol from Sambrook. the question is do I have to use CaCl2 and MgCl2 with molecular biology grade? or biochemical grade is okay? 
thanks before
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#1
boboey
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Posted 05 May 2010 - 07:53 PM
This is my first time trying to make competent cells , and i'm going to adopt the protocol from Sambrook. the question is do I have to use CaCl2 and MgCl2 with molecular biology grade? or biochemical grade is okay? thanks before
, and i'm going to adopt the protocol from Sambrook. the question is do I have to use CaCl2 and MgCl2 with molecular biology grade? or biochemical grade is okay? thanks before
#2
Curtis
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Posted 10 May 2010 - 06:48 AM
Biochemical grade would be sufficient. I think I autoclaved my cacal2 1M.
you can autoclave your glycerol as well, but I never did.
I use invitrogen's protocol for making TOP10
you can autoclave your glycerol as well, but I never did.
I use invitrogen's protocol for making TOP10
#3
biolab12
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Posted 10 May 2010 - 06:50 AM
biochemical grade would be sufficient i guess. I normally sterile filter my solutions. Good luck!
#4
boboey
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Posted 10 May 2010 - 05:13 PM
biolab12, on May 10 2010, 09:50 PM, said:
biochemical grade would be sufficient i guess. I normally sterile filter my solutions. Good luck!
Thank you Curits and Biolab12 for the answers, making me more confident
And Yes, we will surely sterile filter the solutions..
