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CaCl2 for making competent cells


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3 replies to this topic

#1 boboey

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Posted 05 May 2010 - 07:53 PM

This is my first time trying to make competent cells ;) , and i'm going to adopt the protocol from Sambrook. the question is do I have to use CaCl2 and MgCl2 with molecular biology grade? or biochemical grade is okay? :unsure: thanks before

#2 Curtis

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Posted 10 May 2010 - 06:48 AM

Biochemical grade would be sufficient. I think I autoclaved my cacal2 1M.
you can autoclave your glycerol as well, but I never did.

I use invitrogen's protocol for making TOP10

#3 biolab12

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Posted 10 May 2010 - 06:50 AM

biochemical grade would be sufficient i guess. I normally sterile filter my solutions. Good luck!

#4 boboey

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Posted 10 May 2010 - 05:13 PM

biochemical grade would be sufficient i guess. I normally sterile filter my solutions. Good luck!


Thank you Curits and Biolab12 for the answers, making me more confident :ph34r:
And Yes, we will surely sterile filter the solutions..




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