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E. coli 16S or uidA?

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#1 lauren.peterson



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Posted 05 May 2010 - 02:11 PM

Hi all,

I'm a grad student just getting started in my lab. I'm going to be using qPCR to look for E. coli in treated and untreated waters (kind of as a before-and-after type of thing). There are two sets of primers that I could use: a set for the 16S gene or a set for the uidA (beta-D-glucuronidase) gene. I've been looking through papers, and it seems about half of the people doing similar projects use one and the other half use the other with not explination as to why. Any thoughts out there? Is there a reason why you would use one over the other, or is it purely preference?

Thanks for the help. I definitely need it!


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