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Calcium Phosphate Transfection for CV1 CELLS


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2 replies to this topic

#1 JUYASTICK

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Posted 05 May 2010 - 11:44 AM

hI! Someone knows a Calcium Phosphate Transfection Protocol for CV1 CELLS (African Green Monkey kidney cells), it would be very helpful. ;)

Thanks

JUYASTICK

#2 Whitac

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Posted 10 May 2010 - 08:41 PM

hI! Someone knows a Calcium Phosphate Transfection Protocol for CV1 CELLS (African Green Monkey kidney cells), it would be very helpful. :P

Thanks

JUYASTICK


I saw your question & decided to register here.
My biotech class has excellent results with Promega products (kits.) You might find the protocol there http://www.promega.c...m012/tm012.html

We used Promega Fugene for Lipofection and pSV-beta-galactosidase (plasmid with blue detector for selection of transfected CV-1 monkey fibroblast cells - counting efficiency on Weds, but I know it went well.

Edited by Whitac, 10 May 2010 - 09:11 PM.


#3 bsengez

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Posted 18 May 2010 - 05:02 AM

I used lipofectamine 2000 from invitrogen to create stable AGM-CV-1 cell lines and it was successful.

hI! Someone knows a Calcium Phosphate Transfection Protocol for CV1 CELLS (African Green Monkey kidney cells), it would be very helpful. :P

Thanks

JUYASTICK


I saw your question & decided to register here.
My biotech class has excellent results with Promega products (kits.) You might find the protocol there http://www.promega.c...m012/tm012.html

We used Promega Fugene for Lipofection and pSV-beta-galactosidase (plasmid with blue detector for selection of transfected CV-1 monkey fibroblast cells - counting efficiency on Weds, but I know it went well.






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