tcellperson, on May 5 2010, 10:20 PM, said:
I am looking at the data that I received back on my Microarray project and I have this question about the data analysis if anyone can help me understand- Is it possible for a gene to show a fold change and yet be non-significant in terms of p-value ? From what I understand, if a gene exhibits a certain fold change, it has to be significant(with a p-value of less than 0.05). But I am seeing genes on my list which are showing 5 fold or more changed and I am told that they are not necessarily significant!
Can someone please help me if I am missing something?
yes this happens especially when u consider low intensity genes. low intensity genes tend to give false positive results therefore people first set cut off >6 for log2 signal intensity of probe sets.