Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

RNA extraction


  • Please log in to reply
2 replies to this topic

#1 chn09

chn09

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 42 posts
1
Neutral

Posted 05 May 2010 - 05:24 AM

Hello everyone

Ive been trying to extract bacterial total RNA using a Qiagen RNA easy kit. Ive used a 18-20 hr grown culture for RNA extraction. I followed ll the instruction given in the manual but im not getting RNA. Pls help

#2 vladooo

vladooo

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 54 posts
0
Neutral

Posted 05 May 2010 - 11:09 AM

Blame the RNAses.

Use RNAse inhibitors and do your best to avoid RNAses.

#3 pDNA

pDNA

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 493 posts
14
Good

Posted 05 May 2010 - 12:36 PM

What cells are you using?

How do you lyse them?

Try to extract the RNA by using TRIZOL and precipitate it with isopropanol afterwards ...normally this works fine. We collect our cells in 5% phenol solution ...this prevents post-harvest degradation of RNA.

Regards,
p




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.