Posted 05 May 2010 - 05:24 AM
Ive been trying to extract bacterial total RNA using a Qiagen RNA easy kit. Ive used a 18-20 hr grown culture for RNA extraction. I followed ll the instruction given in the manual but im not getting RNA. Pls help
Posted 05 May 2010 - 11:09 AM
Use RNAse inhibitors and do your best to avoid RNAses.
Posted 05 May 2010 - 12:36 PM
How do you lyse them?
Try to extract the RNA by using TRIZOL and precipitate it with isopropanol afterwards ...normally this works fine. We collect our cells in 5% phenol solution ...this prevents post-harvest degradation of RNA.