problems with adherent CHO culture
Posted 04 May 2010 - 08:32 PM
I have a problem with culturing adherent CHO cells, when I defrez a cell batch I can passage it for about a month without any problem, but after that cell growth decreases and they cannot attach to the flask any more and during a week they all are dead.
I never have this problem with other adherent cells, is there any help?
thanks in advance
Posted 11 May 2010 - 07:29 AM
Posted 11 May 2010 - 08:01 AM
CHO cells are relatively hardy- however, once they get to a certain passage number, they are next to impossible to culture. I use CHO-k1 cells and those are great until passage 18-20-- then they start to get funky. Perhaps that would explain your observations.
Posted 11 May 2010 - 09:03 PM
thank you very much for your reply, acctually we bougth the cells from atcc, and I think they should send fresh cells, by the way what do you mean by differentiation of CHO cells? are immortalized cells capable of differentiation?
Posted 12 May 2010 - 07:00 AM