Growing primary neuronal cultures on plastic?
Posted 04 May 2010 - 03:07 PM
Posted 05 May 2010 - 09:29 PM
I don't have much experience though, I got a free sample to try and it worked so I kept using them.
Posted 11 May 2010 - 05:33 AM
People in my lab routinely grow primary neuronal cultures (neonatal mixed glial cultures, embryonic VM and hippocampal cultures) on coated plastic 6/24 well plates to generate cells for PCR or WB, however for the most part we usually plate on coated glass coverslips if we are going to image the cells. We have seen no difference in cells grown on plastic or glass. We using Corning plates at the moment and have used Sarstedt plates in the past, both are fine. Which poly lysine are you using? D or L? Our cells adhere much better on poly D than poly-L treated surfaces. We coat wells with Sigma poly-D-lysine for 30min (although Sigma only advise coating for 5min) followed by 3 dH2O washes, then store the plates at -20 till needed.