siRNA control (scramble seq) causing cell death
Posted 02 May 2010 - 04:19 PM
However, my control scramble sequence is causing more death than any of the knockdown siRNA's.
I know it must be the scramble sequence as a transfection control with Lipofectamine 2000 but no siRNA is fine.
I'm not sure what approach to take next as I can't get any meaningful results from my siRNA's until the scramble is not causing any effects. Should I try a different transfection reagent? I have tried two different concentrations of siRNA and get a similar result with both concentrations.
Thanks for any help!
Posted 02 May 2010 - 06:48 PM
Even there is no significant homology with other sequences, it is known that some siRNA sequences could have sequence non-specific cytotoxic or immunostimulating effects. If that is the case you should switch to another control siRNA.
Posted 05 May 2010 - 09:34 PM