Hi,
I'm trying to clone a PCR product into a suicide vector which would hopefully inactivate a specific gene through recombination. My vector has 2 multiple cloning sites... Does anyone know if the cloned gene orientation matters? Should it come before or after the antibiotic marker?
Thanks !
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#2
phage434
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Posted 02 May 2010 - 05:52 AM
It shouldn't matter. How large are your recombination sites?
#3
fuso-girl
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Posted 02 May 2010 - 10:18 PM
phage434, on May 2 2010, 06:52 AM, said:
It shouldn't matter. How large are your recombination sites?
Hi,
About 1kb. I plan to clone different restriction sites for the ends to ensure the correct orientation in the vector, but I just wasn't sure about the antibiotic marker.
