Posted 09 August 2002 - 02:09 AM
We're using GFX Amersham's kit to purify DNA from solution and agarose gel bands. Although sometimes it works right, since few weeks to nowadays we're having some trouble. When we run the purified band in a new agarose gel, it appears a new DNA band, almost of half the weight of that purified, and our band becomes only slightly visible. We don't know if it's denaturation, secondary structure o contamination, but it happened to more than one person. It happens with all band sizes tryied. We don't know a possible explanation. Did it happened to anybody? Do you know a possible explanation, and solution?