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what is 0.5 x TSB?


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#1 archercr

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Posted 30 April 2010 - 09:23 AM

What is 0.5 x TSB? my guess is a diluted form of TSB. I've also seen methods call for 1 x TSB.

Other question; if I purchase TSB media already made up and I need to add something (like powdered saponin for neutralization), can I re-autoclave it? I'm just thinking of damaging the nutrients if autoclaved too many times. Or, maybe it's better to just dissolve the powder in water and sterile-filtrate it...thoughts?

#2 gebirgsziege

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Posted 02 May 2010 - 10:45 PM

0.5 means usually only half the amount of nutrients (so diluted 1:2).

I would filter-sterilise the additives. Otherwise sugar burns or metals can form precipitates etc.
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