I have tried numerous methods with EDTA where in the colon is cut longitudinally and then incubated in 5-30mM EDTA shaking at 37 degrees for 15 minute shakes.
I've also tried to evert the colon onto a glass rod and then shake in EDTA.
The fractions that come off do not seem to yield any IECs or if they do, they are all dead (by Trypan blue stain)
If there is someone out there who has isolated IECs, I would be immensely grateful if you could provide me with a detailed protocol.
Edited by retrograde, 30 April 2010 - 04:09 AM.