Hi all,
I used dynabeads protein G for with anti-rabbit antibody to immunopurify my protein. but, i couldnt' immunoprecipitate!
I'm wondering if its question of protein G, and i want to try Protein A. I found protein A-agarose aliqot and i want to try to check if i could pull down!
i would like to ask if protein A-sepharose is better than A- agarose in my case (to purify my protein from cerebral lysate)!!
thanks for help
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3 replies to this topic
#2
mdfenko
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Posted 30 April 2010 - 06:55 AM
sepharose is ge healthcare's (nee pharmacia) brand name for agarose. protein a-sepharose and protein a-agarose are virtually the same.
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genius does what it must
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#3
jasmina
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Posted 30 April 2010 - 07:02 AM
ah ok,!! i see!
because one collegue that A-sepharose is more used and better than agarose!
thanks for your answer!
because one collegue that A-sepharose is more used and better than agarose!
thanks for your answer!
#4
mdfenko
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Posted 30 April 2010 - 07:07 AM
well, there are some aging and quality control issues but for your purpose it should make no significant difference (i prefer sepharose to generic agarose).
Edited by mdfenko, 30 April 2010 - 07:08 AM.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
