2 replies to this topic

[kaposi_pc]

Dear all,

I wish to do p53 related studies and I was wondering if HEK 293 cells contain fucntional Adenovirus proteins like E1A or E1B?If so,do they inhibit p53 in these cells?Would this affect my readout of p53-reporter based experiments?

Thanks!!

[bob1]

They contain the genome of adenovirus, but I don't know if it is expressed or not.  I suspect not.  The p53 pathway is inhibited in some fashion in most if not all cancer and immortal cell types.

[sara.r]

kaposi_pc, on Apr 29 2010, 01:56 AM, said:

Dear all,

I wish to do p53 related studies and I was wondering if HEK 293 cells contain fucntional Adenovirus proteins like E1A or E1B?If so,do they inhibit p53 in these cells?Would this affect my readout of p53-reporter based experiments?

Thanks!!

hi
yes they express functional e1a/e1b and that is why expression from cmv promoter is much higher in these cells compared to other cells (e1a transactivates cmv promoter)