Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

ELISA doubts


  • Please log in to reply
1 reply to this topic

#1 wntiong

wntiong

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 58 posts
0
Neutral

Posted 28 April 2010 - 10:24 PM

Hi i need help on ELISA about:

1. I going to use tissue lysate for ELISA, but if i previously use evan blue to stain my tissue, will this affect the ELISA reading?

2. Have u heard about NGF ELISA kit? What brand would you recommend?

3. Most kits i found recommend to use tissue/cell line cultures for NGF detection, because the plasma and sera contain IgG. How does this affect the ELISA performance?

Need your help.

Thanks.

#2 sgt4boston

sgt4boston

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 276 posts
1
Neutral

Posted 30 April 2010 - 07:02 AM

Spike the stain into your calibrators to determine the level that may inhibit the curve.

Manufacturers should be able to provide you with details as to how sera and plasma interfere in their products. You should request information as to their kit performance with interfering substances. Perhaps they have detailed information as to levels / concentrations of sera/plasma/IgG that interfere.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.