Bisulfite Conversion Efficiency
Posted 28 April 2010 - 08:09 PM
Also I am trying to optimize bisulfite conversion efficiency of FFPE samples....does anyone have experience with this?
Posted 18 May 2010 - 06:00 PM
Efficiency is then measured by cloning and sequencning methods where non-CpG C's sequenced are counted across all the clones sequenced.
Note this is only conversion efficiency of the region you are interested in and PCR amplified, and not necessarily a measure of efficiency across the whole genome. There are some sequence contexts where conversion could be inhibited by GC-content and secondary structures.
All comments and communication are my own personal ones, and are not tied to any of my affiliations.