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Immunohistochemistry for nNOS oreNOS?

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#1 anonymous



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Posted 20 February 2001 - 10:00 PM


I am wondering if anyone has a working protocol for immunofluorescence labelling of nNOS or eNOS on spleen tissue.I have tried to label nNOS or eNOS on rat skeletal muscle cryostat sections using a published protocol.The results were not very obvious. The labelling on skeletal muscle is my positive control.Therefore, before I do the labelling on splenic tissues, I want to ensure the antibodies be working on my positive controls first.Thanks!

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