Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Retro-virus work

  • Please log in to reply
1 reply to this topic

#1 SciCell



  • Active Members
  • PipPipPipPipPip
  • 38 posts

Posted 28 April 2010 - 04:09 PM

Hello all,

Recently I took up a new job where I have to do transfections into the packaging cell lines and generate retroviruses followed by infecting target cell lines, characterizing and generating stable cell lines. After reading the concerned literature, I was just wondering how safe it is to work in this job?
I worked in with various cell lines in the past including primary cells and human embryonic stem cells, and also transfections and transductions using lenti virus but never retro virus. Do you have any suggestions?


#2 bob1


    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,482 posts

Posted 28 April 2010 - 05:34 PM

Lentivirus is a retrovirus - HIV is a lentivirus! So long as you follow appropriate biological precautions (work in a hood, wash hands etc.) and have good technique, you shouldn't have an problems.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.