very high molecular weight plasmid
Posted 28 April 2010 - 05:57 AM
Posted 28 April 2010 - 06:46 AM
Non-linearised DNA will run slower than linear DNA and hence look bigger in size compared to a DNA ladder.
hi everyone. i recently prepared maxiprep of a plasmid with gfp. this plasmid was commercially available and just transformed them and prepared maxiprep. the plasmid i purified has an OD of 1.9 so i think its okay however when i checked it in agarose, the size is very high, higher than the first line of the dna marker. I expect that the MW of my plasmid would be around 5-6Kb only.
Posted 28 April 2010 - 07:07 AM
Posted 28 April 2010 - 08:11 AM
Posted 28 April 2010 - 09:03 AM
Posted 28 April 2010 - 05:02 PM
You can not get an estimate of a circular DNA's size by comparing it to a linear DNA ladder...
oh thank you thank you. i purified it by etbr-phenol-chloroform after maxiprep. are there any suggestion how to confirm if my plasmid i run on the gel and purified is correct. sequencing?
Posted 28 April 2010 - 05:06 PM