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flagella stain


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#1 analia

analia

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Posted 28 April 2010 - 05:04 AM

Hi! I want to stain flagella from Xhantomonas.
There is a protocol in which I have to make a solution with phenol 5% in water, AlKS2O8 and crystal violet in etoh.
My problem is that I don't know how to make phenol 5% in water.
Anyone can help me?
Is it just to melt 5 g phenol and mix them with 100 ml water? Does it really mix??
Can I prepare it from melted phenol equilibrated in water or tris?

Thanks a lot.
Analía

#2 gebirgsziege

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Posted 29 April 2010 - 04:15 AM

I would prepare in TE, otherwise the Phenol will degrade quickly. But I think to rember that you need to prepare the solutions fresh everytime....
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