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phenol 5% help!


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#1 analia

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Posted 28 April 2010 - 04:53 AM

Hi! I would like to know how to make phenol 5% in water. It is for flagella stain
Thanks!
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#2 mdfenko

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Posted 28 April 2010 - 05:58 AM

w/v: take 5 gm phenol crystals and add water to 100 ml.
talent does what it can
genius does what it must
i do what i get paid to do

#3 analia

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Posted 28 April 2010 - 06:06 AM

w/v: take 5 gm phenol crystals and add water to 100 ml.


Do I melt phenol first?
Does it really mix? The only phenol I have ever prepare is for DNA extraction. And it becames a two-phase solution. That is why my doubts.

Thanks!
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#4 mdfenko

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Posted 28 April 2010 - 06:13 AM

the phenol will dissolve in the water (1gm/15ml water)

Edited by mdfenko, 28 April 2010 - 06:15 AM.

talent does what it can
genius does what it must
i do what i get paid to do




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