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How to store wet dialysis membranes once opened?


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#1 PhD

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Posted 27 April 2010 - 11:54 PM

Hello there,

ive bought a wet dialysis membrane and I was wondering what to do with it once openened? I only need a piece at a time and I guess it has to stay wet. Shall I put it in a becker with water and keep it in the fridge? Sorry for the silly question but its expensive and I dont want to damage it!


thanks!!

#2 rhombus

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Posted 28 April 2010 - 02:29 AM

Hello there,

ive bought a wet dialysis membrane and I was wondering what to do with it once openened? I only need a piece at a time and I guess it has to stay wet. Shall I put it in a becker with water and keep it in the fridge? Sorry for the silly question but its expensive and I dont want to damage it!


thanks!!



If the Dialysis tubing you purchased came dry, say in a roll, it can be left that way indefinetely. When I Dialyse Foetal Calf Serum, I cut a piece of tubing to a desired amount, then hydrate it overnight in Sterile Distilled water. Normally I do not store Dialysis tubing in Distilled Water, as I only cut off the desired amount.

Hope this answers your question

Kindest regards

Uncle Rhombus

#3 K.B.

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Posted 28 April 2010 - 05:37 AM

You may store wet dialysis membrane (tubing) in distilled water with some antibacterial agent eg. sodium azide or merthiolate (some manufacturers recommend ethanol).

I rehydrate dialysis tubing for 15-30 minutes in hot water (80-100 degrees C).

#4 phage434

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Posted 28 April 2010 - 02:21 PM

Kathon is also an excellent low-toxicity preservative for items such as this.

#5 PhD

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Posted 29 April 2010 - 01:25 AM

Thanks guys!

In the end I took it out of the bag and poured the sodium azide in a becker that was just wide enough for the roll. Then added a bit more water so it would all be covered, wrapped it with parafilm and put in the fridge.


Used it yesterday and it worked great!




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