Does anybody, I di my DNA extraction using salting out method. Unfortunately, I forgot to add 20% of SDS ath the fvery first step (together with Nuclei Lysis buffer and Proteinase K). I just realized that i did not added that SDS when I found ot there were no DNA 'cotton wool' form when adding absolute cold ethanol. How to resolve this problems becaue the samples is very precious (patients).
CAn I re-do the expreiment with that added ethanol cells from the benning step?
Edited by pearlwhite, 27 April 2010 - 05:32 PM.