Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Troubleshooting on DNA Extraction-Salt extraction


  • Please log in to reply
1 reply to this topic

#1 pearlwhite

pearlwhite

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 27 April 2010 - 05:31 PM

Hello,

Does anybody, I di my DNA extraction using salting out method. Unfortunately, I forgot to add 20% of SDS ath the fvery first step (together with Nuclei Lysis buffer and Proteinase K). I just realized that i did not added that SDS when I found ot there were no DNA 'cotton wool' form when adding absolute cold ethanol. How to resolve this problems becaue the samples is very precious (patients).

CAn I re-do the expreiment with that added ethanol cells from the benning step?

Edited by pearlwhite, 27 April 2010 - 05:32 PM.


#2 Adrian K

Adrian K

    Legendary Graduate Beggar

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 708 posts
28
Excellent

Posted 07 June 2010 - 09:37 PM

You can just use it directly. If your salting out method involves boiling, you probably might already have some DNA in it.
Cotton wool is not always necessary.
Expecting the world to treat you fairly because you are a good person is like expecting the lion not to attack you because you are a vegetarian.

..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...

"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong

"It's all just DNA. Do it."---phage434




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.