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Troubleshooting on DNA Extraction-Salt extraction

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#1 pearlwhite



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Posted 27 April 2010 - 05:31 PM


Does anybody, I di my DNA extraction using salting out method. Unfortunately, I forgot to add 20% of SDS ath the fvery first step (together with Nuclei Lysis buffer and Proteinase K). I just realized that i did not added that SDS when I found ot there were no DNA 'cotton wool' form when adding absolute cold ethanol. How to resolve this problems becaue the samples is very precious (patients).

CAn I re-do the expreiment with that added ethanol cells from the benning step?

Edited by pearlwhite, 27 April 2010 - 05:32 PM.

#2 Adrian K

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Posted 07 June 2010 - 09:37 PM

You can just use it directly. If your salting out method involves boiling, you probably might already have some DNA in it.
Cotton wool is not always necessary.
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