Posted 27 April 2010 - 10:18 AM
My question is this: did I trash my DNA or do you think my mutagenesis actually worked like the gel suggests and go ahead with the transformation?
Posted 27 April 2010 - 10:24 AM
Posted 27 April 2010 - 10:31 AM
Posted 27 April 2010 - 04:21 PM
I would say go ahead and do the transformation. If the transformed cells grow, pick a single colony and do a miniprep, sequence the plasmid. The cost is relatively small and the result can be really convincing.
Why not program the thermal cycler to automatically go into the cycling after the 5-min 95C incubation?