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is there an antibody elution buffer that allows cultured RGCs to survive?


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#1 karadril

karadril

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Posted 27 April 2010 - 07:02 AM

Hi

I was wondering if it is possible to use a buffer that disrupts antigen-antibody binding whilst not killing or damaging cultured RGCs.
The reason for this is because I would like to use magnetic beads with Thy1 antibody to select for RG cells, and re-use the beads.
I have no experience in cell culture or antibody elution, but I suspect that the conditions required to separate antigens from antibodies might be too harsh for cells to survive. Still, it would be a nice surprise if this would be possible :rolleyes: .




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