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KpnI Restriction enzyme


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#1 maxmix

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Posted 27 April 2010 - 05:28 AM

hi i am new here:
I am trying to identify this SNP: Gly16Arg of beta2 adrenergic receptor gene.
I used this primer: F: 5-AGCCAGTGCGCTTACCTGCCAGAC-3
and the reverse primer:
3-CATGGGTACGCGGCCTGGTGCTGCAGTGC-5.
Vesna D.ET,AL2002.

And The Restriction enzyme KpnI.

so how I can know the polymorphism as wild type or homo zygote SNP or hetero zygote.
thnx

#2 Curtis

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Posted 27 April 2010 - 10:01 AM

I don't see any GGTACC in your primer sequence so I assume your RE site must be within your PCR product sequence. This depends how many bp your product is also. but if you digest and you see 2 or more bands on the gel compared to your original PCR product then the KpnI site must be there.

Did I help?




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