DNA contamination in RNA samples
Posted 27 April 2010 - 03:37 AM
I have isolated RNA from very precious frozen samples by using Trizol, but i have DNA contamination which i found out by using no RT. Is there any DNase treatment protocol i can use to eliminate the DNA from my RNA samples with minimum RNA loss. Thanx.
Posted 27 April 2010 - 04:31 AM
But I don't understand how you figured out you have DNA in your sample. no RT? what is that?
When extracting RNA from Eukaryotic sample you would normally end up with DNA contamination.
Posted 27 April 2010 - 01:44 PM