autoclaving for a second time
Posted 27 April 2010 - 02:38 AM
would it be bad to autoclave a broth again?
I have a broth, but I need agar, so I was thinking of putting some agar in the bottle and then autoclaving it again.
Is this ok or?
Posted 27 April 2010 - 06:12 AM
Posted 27 April 2010 - 04:25 PM
Posted 28 April 2010 - 09:47 PM
Myself have done that twice (and will make sure it'll never be a routine) and things grow well. They get a lil darker that's all. Grows well for me.
But then, I did that with a broth and I'll spare you the reason why I did that. Not sure about agar though since, like Protein Work puts it, if pH changes, it'll affect the agar after cooling.
Posted 29 April 2010 - 04:05 AM
If you are working with a standard medium and just want to keep your microbes growing and your microbes do not need some special nutrients its no problem if the medium is autoclaved for a second time. But if you need the medium for an experiment crucial for your research, make a fresh one to be on the save side.