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Problem with Endotoxin removal


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#1 pattadons

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Posted 26 April 2010 - 09:22 PM

Hello, I am new for Bioforum. I have a problem about endotoxin removal from recombinant protein with Triton X 114. I incubated the mixture at 4C for 30min and 37 C for 10 min, then followed by centrifuged at 20,000 xg for 10min. However the solution was not separated. It was turbid and looked like a number of lipid particles were still floating. I would appreciate if anyone could suggest me how to resolve this problem.

Many thanks,

Patt

Edited by pattadons, 26 April 2010 - 09:32 PM.


#2 mdfenko

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Posted 27 April 2010 - 06:58 AM

have you considered using endotrap?
talent does what it can
genius does what it must
i do what i get paid to do

#3 AnetteM

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Posted 13 May 2013 - 04:39 AM

I have tried using EndoTrap and Triton X114 but both have failed in removing endotoxins. Does anyone have a better suggestion?

/Anette

#4 phage434

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Posted 13 May 2013 - 04:54 AM

You could try the new E. coli cells from Lucigen that claim to have genetically removed the endotoxin production.




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