7 replies to this topic

[forrestlin]

Hi, everybody. I want to construct a cell model about Alzheimer's Disease, but I am not very familiar with this aspect. So, could you please tell me the best cel model for AD? such as which cell line is best for A-beta transfection?
Thanks so much.

[BioSaint]

CHO probably.. check out Shankar et al for the natural oligomers.. Selkoe and collaborators

forrestlin, on Apr 27 2010, 03:55 AM, said:

Hi, everybody. I want to construct a cell model about Alzheimer's Disease, but I am not very familiar with this aspect. So, could you please tell me the best cel model for AD? such as which cell line is best for A-beta transfection?
Thanks so much.

[mickey]

forrestlin, on 26 April 2010 - 06:55 PM, said:

Hi, everybody. I want to construct a cell model about Alzheimer's Disease, but I am not very familiar with this aspect. So, could you please tell me the best cel model for AD? such as which cell line is best for A-beta transfection?
Thanks so much.

You could use swedish mutant APP or C99+Met combinant peptide plasmid. HEK293 or HEK 293T is OK.

Good luck.

[sera_tonin]

forrestlin, on 26 April 2010 - 06:55 PM, said:

Hi, everybody. I want to construct a cell model about Alzheimer's Disease, but I am not very familiar with this aspect. So, could you please tell me the best cel model for AD? such as which cell line is best for A-beta transfection?
Thanks so much.

in the past i've used HEK293T cells for Abeta transfection, and it worked well - but i'm not in that field anymore, so i don't know what the current consensus is.

[scolix]

people use different cell lines and make stables. HEK293, neuroblastoma (SHSY5Y) etc.

[thean]

I have read journals on researchers using IMR32 cell lines for basic research studying the protective effects of plant bioactives on amyloid beta peptide insult of this cell line.

However I find this cell line  a loosely adherent cell line and always detach itself from plates while changing medium even when we use collagen coated plates.

Any suggestions?

[knuf]

thean, on 20 January 2011 - 11:38 PM, said:

I have read journals on researchers using IMR32 cell lines for basic research studying the protective effects of plant bioactives on amyloid beta peptide insult of this cell line.

However I find this cell line  a loosely adherent cell line and always detach itself from plates while changing medium even when we use collagen coated plates.

Any suggestions?

Yes, this is a known characteristic of IMR32 cells. Using warmed media helps with this, but only slightly. Treating them with BrdU or dbcAMP will cause them to differentiate and take on a neuronal morphology, and they also become more adherent.

[TaveiraM]

Hi everybody!

I already evaluated the AChE and BChE inhibition capacity of different plant extracts.
Now I want to test the most promising ones in SH-SY5Y cell lines.

Apart from the cytotoxicity evaluation which assays you suggest to do to evaluate the Alzheimer's capacity?

Best regards