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Alzheimer's cell model


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7 replies to this topic

#1 forrestlin

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Posted 26 April 2010 - 06:55 PM

Hi, everybody. I want to construct a cell model about Alzheimer's Disease, but I am not very familiar with this aspect. So, could you please tell me the best cel model for AD? such as which cell line is best for A-beta transfection?
Thanks so much.

#2 BioSaint

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Posted 22 June 2010 - 03:25 AM

CHO probably.. check out Shankar et al for the natural oligomers.. Selkoe and collaborators

Hi, everybody. I want to construct a cell model about Alzheimer's Disease, but I am not very familiar with this aspect. So, could you please tell me the best cel model for AD? such as which cell line is best for A-beta transfection?
Thanks so much.



#3 mickey

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Posted 28 July 2010 - 06:22 PM

Hi, everybody. I want to construct a cell model about Alzheimer's Disease, but I am not very familiar with this aspect. So, could you please tell me the best cel model for AD? such as which cell line is best for A-beta transfection?
Thanks so much.


You could use swedish mutant APP or C99+Met combinant peptide plasmid. HEK293 or HEK 293T is OK.

Good luck.

#4 sera_tonin

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Posted 30 July 2010 - 12:00 PM

Hi, everybody. I want to construct a cell model about Alzheimer's Disease, but I am not very familiar with this aspect. So, could you please tell me the best cel model for AD? such as which cell line is best for A-beta transfection?
Thanks so much.


in the past i've used HEK293T cells for Abeta transfection, and it worked well - but i'm not in that field anymore, so i don't know what the current consensus is.

#5 scolix

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Posted 29 December 2010 - 11:40 AM

people use different cell lines and make stables. HEK293, neuroblastoma (SHSY5Y) etc.

#6 thean

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Posted 20 January 2011 - 11:38 PM

I have read journals on researchers using IMR32 cell lines for basic research studying the protective effects of plant bioactives on amyloid beta peptide insult of this cell line.

However I find this cell line a loosely adherent cell line and always detach itself from plates while changing medium even when we use collagen coated plates.

Any suggestions?

#7 knuf

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Posted 21 January 2011 - 10:20 AM

I have read journals on researchers using IMR32 cell lines for basic research studying the protective effects of plant bioactives on amyloid beta peptide insult of this cell line.

However I find this cell line a loosely adherent cell line and always detach itself from plates while changing medium even when we use collagen coated plates.

Any suggestions?


Yes, this is a known characteristic of IMR32 cells. Using warmed media helps with this, but only slightly. Treating them with BrdU or dbcAMP will cause them to differentiate and take on a neuronal morphology, and they also become more adherent.

#8 TaveiraM

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Posted 22 September 2011 - 07:04 AM

Hi everybody!

I already evaluated the AChE and BChE inhibition capacity of different plant extracts.
Now I want to test the most promising ones in SH-SY5Y cell lines.

Apart from the cytotoxicity evaluation which assays you suggest to do to evaluate the Alzheimer's capacity?

Best regards




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