2 replies to this topic

[iamdhan]

Hi,

I am gonna make buffer according to this protocol: http://www.lamondlab...inisolation.pdf

It is for experiment called "Chromain Isolation by small-scale biochemical Fractionation"

Buffer A
10 mM HEPES, pH 7.9
10 mM KCl
1.5 mM MgCl2
0.34 M Sucrose
10 % Glycerol
1 mM DTT
Protease inhibitor cocktail

Buffer B
3 mM EDTA
0.2 mM EGTA
1 mM DTT
Protease inhibitor cocktail

I am wondering where I can keep those buffer; should I leave it at room temperature or in the fridge??

Thx!

[pcrman]

keep them in the fridge and add protease inhbitors right before use.

[MarcinK]

Hi,
I'm newbie about chromatin isolation. Can someone tell me what kind of pellet should expect at the end of the protocol described above. Smooth or fluffy? How to measure the concentration of isolated chromatin? Typical 260/280 nm?
With regards
Marcin from poland