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downshifted band


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4 replies to this topic

#1 cellgene

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Posted 25 April 2010 - 12:20 PM

When I load a cell lysate on a gel and do western blot against my protein of interest, I see a band at the right size(56kDa) and another that's about 10kDa lower.

When I perform an siRNA knockdown the lower band also disappears.

I know many modifications causing an upshift, but what can be causing any downshifts?

Thanks for the suggestions in advance

#2 bob1

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Posted 25 April 2010 - 04:28 PM

Potentially cleavage of a fragment.

#3 Inmost sun

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Posted 29 April 2010 - 11:22 AM

When I load a cell lysate on a gel and do western blot against my protein of interest, I see a band at the right size(56kDa) and another that's about 10kDa lower.

When I perform an siRNA knockdown the lower band also disappears.

I know many modifications causing an upshift, but what can be causing any downshifts?

Thanks for the suggestions in advance


proteolysis or splice variation or loss of posttranslational modifications

#4 cellgene

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Posted 05 May 2010 - 04:37 AM

thank you
i couldn't find any cleavage sites bioinformatically- but of course it still can be

So no modification causes the protein to run lower?

#5 mdfenko

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Posted 05 May 2010 - 06:34 AM

thank you
i couldn't find any cleavage sites bioinformatically- but of course it still can be

So no modification causes the protein to run lower?

an example, carboxypeptidases will remove one amino acid at a time, no specific cleavage site other than it be the carboxy terminus.

cleavage can be a ptm.
talent does what it can
genius does what it must
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