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PBS for ELISA


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3 replies to this topic

#1 Avivit

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Posted 25 April 2010 - 03:30 AM

hi,

Is there a difference between PBS-/- and PBS +Mg+Ca when performing an elisa assay?

Thanks,

Avivit

#2 klinmed

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Posted 25 April 2010 - 11:11 PM

hi,

Is there a difference between PBS-/- and PBS +Mg+Ca when performing an elisa assay?

Thanks,

Avivit

You could use either, but it is normal to use PBS without Ca/Mg. One exception is if your antibody is dependent on Ca for binding (eg the anti-FLAG monoclonal M1).

Hope this helps.

#3 Avivit

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Posted 26 April 2010 - 12:06 AM

Thanks that what we thought but weren't sure...

#4 sgt4boston

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Posted 26 April 2010 - 04:19 AM

or it is needed for the enzyme to be active.




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