I have a BIGGGG problem with my RT-PCR!!! I have made a TaqMan microna assay (AB), using the sno292 as endogenous control. I know this is a good control for my cells, but, in the last RT-PCR it is very variable among the samples (until 3 Ct of difference...). What does it happen? RNA degradation?However, can I analyze this RT-PCR, making a DDCt study, or not??
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variable endogenous control
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