Hi.I'm trying to stain for Nuclear steroid-type receptors in snap-frozen, ethanol fixed sections with polyclonal & monoclonal antibodies, but I can't seem to get good clear expression.Is there a more appropriate immunohistochemistry technique for staining nuclear receptors?thanks
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1 reply to this topic
Posted 20 August 2001 - 09:00 PM
I would try perfused-fixed tissue, preferably 4% paraformaldehyde. Antigenicity of steroid receptors is easily lost in inadequately fixed tissue. Hope this reply isn't too late in the day - I only just accessed the site. Cheers