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Antibody for Fusion Protein


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#1 slav

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Posted 23 April 2010 - 02:46 AM

Hi everyone, it may well be obvious to someone, but, when I want to target fusion proteins, do I need to search for TWO antibodies against both parts of the fusion?

For example, I have wt of protein X and Y, and I have one copy of fusion between them X`-Y`. How can I validate the quantity of both wt and fusion form?

Finally, I want to use commercially available antibodies and don`t want to raise my own.

Thanks for any advice.
best

#2 Inmost sun

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Posted 29 April 2010 - 11:17 AM

Hi everyone, it may well be obvious to someone, but, when I want to target fusion proteins, do I need to search for TWO antibodies against both parts of the fusion?

For example, I have wt of protein X and Y, and I have one copy of fusion between them X`-Y`. How can I validate the quantity of both wt and fusion form?

Finally, I want to use commercially available antibodies and don`t want to raise my own.

Thanks for any advice.
best


what immunological method will be used, preferentially ELISA or immunoblot?; you need two antibodies for semi-quantification; more precise is MS/MS

#3 slav

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Posted 30 April 2010 - 01:08 AM

I want to do Western blot, should be most suitable.

MS/MS? What do you mean? Mass spectometry?

Edited by slav, 30 April 2010 - 01:09 AM.





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