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Mouse eyes fixation and cryosectioning


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#1 Andromachi

Andromachi

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Posted 23 April 2010 - 02:10 AM

Hi!

I read that you can use Davidson's fixative for eye fixation (it is said to be better compared to PFA, also for the staining). My question is how do you treat the eyes after fixing them? Could you use sucrose for cryoprotection and then cut it in cryosections? Or you have to embed them in paraffin? I would like to prepare some cryosections, but I don't know whether I should use Davidson's fixative or PFA.
The second question is the time that you fix the eyes. What is the optimal time that you keep them in the fixative? Does it depend on the age of the mouse or is it the same for different ages (as the tissue remains the same more or less).

Thank you!




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