1 reply to this topic

[sam_boooko]

Hi
I was wondering if someone could help me. Everytime I run my protein samples on 2-D gels and silver stain these gels I get this large blob at the base of the gel (see attached picture). I use GE IEF strips and ampholytes and Invitrogen pre-cast gels. I also use Invitrogen LDS sample buffer. I take great care not to touch the gels before staining.
Any help appreciated.
sam

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[Prep!]

sam_boooko, on Apr 22 2010, 11:31 PM, said:

Hi
I was wondering if someone could help me. Everytime I run my protein samples on 2-D gels and silver stain these gels I get this large blob at the base of the gel (see attached picture). I use GE IEF strips and ampholytes and Invitrogen pre-cast gels. I also use Invitrogen LDS sample buffer. I take great care not to touch the gels before staining.
Any help appreciated.
sam

may be u shud try sum sample clean up kits available in the market!!