To compare the altered expression of signaling molecules in obesity, I want to use small (i.e.: "normal" or non-hypertrophic) & large (i.e.: hypertrophic) adipocytes, and I need a protocol for either separating them or inducing triglyceride storage in subculture.
I've read techniques where primary adipocytes were separated by size using buoyancy, but I am hearing that will be very difficult... Anyone have any experience with this? I don't have (immediate) access to FACS...how about separating them by passing them through a membrane with pores of a particular size?
My assumption is that it would be possible to manipulate conditions in the culture to induce hypertrophy...maybe insulin? Can anyone point me to a protocol (or a study that did it) for achieving differential triglyceride storage?
Inducing Hypertrophy in Adipocytes and/or Separation by Size
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