No replies to this topic

[PJRoehrich]

To compare the altered expression of signaling molecules in obesity, I want to use small (i.e.: "normal" or non-hypertrophic) & large (i.e.: hypertrophic) adipocytes, and I need a protocol for either separating them or inducing triglyceride storage in subculture.  

I've read techniques where primary adipocytes were separated by size using buoyancy, but I am hearing that will be very difficult...  Anyone have any experience with this?  I don't have (immediate) access to FACS...how about separating them by passing them through a membrane with pores of a particular size?

My assumption is that it would be possible to manipulate conditions in the culture to induce hypertrophy...maybe insulin?  Can anyone point me to a protocol (or a study that did it) for achieving differential triglyceride storage?

Thanks!!
Peter