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NP40 or Triton as membrane protein detergent


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#1 jasmina

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Posted 22 April 2010 - 02:33 AM

Hi,
Im using ripa buffer to solubilize brain membrane protein.
I checked ripa components of others!, im wondering if NP40 is better than tritonX-100 for my case.
If someone knows about detergent efficient for membran protein solubilization, please, let me know
thanks

#2 DaveD

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Posted 29 April 2010 - 09:10 PM

Hi Luciana,

check out the NP40 vs. TritonX-100 messy area of nomenclature.

Although NP40 is a good general detergent to solubilize membrane proteins, you often have to optimize the solubilization conditons. Detergent is only one factor. There's also time, temperature and the solution properties (salt, pH etc.).
In the end it depends, what you're planning to do with the extracted membrane protein sample. If the goal is to get the target protein onto a western you could go with 15%SDS (plus 5% dodecylmaltoside may be a good idea). If you want to carefully solubilize the target protein in its functional state, then you may need to optimize. This can be done with a screen of detergents and extraction conditions.
Check with Dilyx' solubilization screens - they'll be offering a membrane protein solubilization screen.

Cheers,
David


Hi,
Im using ripa buffer to solubilize brain membrane protein.
I checked ripa components of others!, im wondering if NP40 is better than tritonX-100 for my case.
If someone knows about detergent efficient for membran protein solubilization, please, let me know
thanks






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