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RT-PCR Gel


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#1 ColoSarah

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Posted 21 April 2010 - 03:05 PM

I'm having some issues running a gel. My product is ~80 bp so I am running it on a 2% gel at 90-100 V for 40 minutes. The ladder isn't getting good separation and my product appears fuzzy. Does anyone have any suggestions? Thanks!

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  • NQO1_042110_Primer_1_Lung_and_Spleen_RT_PCR.jpg


#2 spring

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Posted 21 April 2010 - 09:06 PM

my products  is  206bps,3% gel.The ladder got good separation. you may try to increase your gel concentration.try 3% gel or higher.




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