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#1 RosalieZ

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Posted 21 April 2010 - 11:52 AM

Hello there,

I have some questions concerning ORFs
Start y x z stop
--------------------------------------------------------

1. an ORF in prokaryotes is characterized by having a start and stop codon in the ends. I have an ORF cloned in a vector in E.coli. Following homologystudies in a database I get the idea that x can be part of the active site of the encoded enzyme.
a. Describe why you would come to this conclusion based on the sequences alone.
b. homologystudies show that ever in y a pointmutation has occurred. From what does this shows and what type of mutations could these be?
c. I want to make a (point)deletionmutation in z. Describe a method with a double PCR.
d. To confirm that z is mutated I will sequence the vector. What primers can I use?

I hope to get some answers!

Rosalie.

#2 leelee

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Posted 21 April 2010 - 08:38 PM

This sounds like a homework question- and as such should have been posted in the dedicated section for such questions.
We don't tend to just give answers on here, but if you give us your thoughts then we can help point you in the right direction.......

#3 RosalieZ

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Posted 25 April 2010 - 11:41 AM

This sounds like a homework question- and as such should have been posted in the dedicated section for such questions.
We don't tend to just give answers on here, but if you give us your thoughts then we can help point you in the right direction.......


I'm sorry, just started out here..

#4 leelee

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Posted 27 April 2010 - 11:15 PM

No need to be sorry- I wasn't having a go at you, just letting you know how it works.
Why don't you tell us what you were thinking the answers are, or might be, or even just what you know about each question and then we can help you <_<




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