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RNA immunoprecipitation...HELP

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#1 quinner



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Posted 21 April 2010 - 07:05 AM

Hi all,

I really hope someone can help me-I've been studying a really interesting RNA binding gene whose targets are not particularly well established.

I would really like to do a RIP-SEQ on it however my experience of Protein work/IP is extremely limited and unfortunately no one in my lab is much help either!. For that reason i was planning on using a kit but i would like some advice even if it is just to say I'm wasting my time on too complicated an experiment for someone with no experience.

I'm mostly confused about the best ways to check if the efficiency of the IP and that IP has worked correctly given that I don't know the binding targets.

if anyone has an advice-I would really appreciate it.

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