Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

degraded cDNA from immunohistochemically stained tissues

  • Please log in to reply
1 reply to this topic

#1 Mathias Droll

Mathias Droll


  • Members
  • Pip
  • 5 posts

Posted 06 February 2002 - 01:11 AM

RNA isolation from immunohistochemically stained frozen tissues with subsequent RT-PCR works well.
However, after freezing of cDNA the PCR doesn`t work anymore.
There is no cDNA detection anymore.

any suggestions?

#2 boris pufong

boris pufong


  • Members
  • Pip
  • 4 posts

Posted 12 February 2002 - 06:35 AM

cDNA is quite stable at -20 -80 degrees for at least six months. find out if they has been any change in your PCR reagents especially the Taq making sure it stillhas its properties. Also continues thaw and freeze cycles of the cDNA could lead to degredation
good luck

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.