Blood specimen banking for biomarker
Posted 21 April 2010 - 01:28 AM
I am a microbiologist. I am trying to find a method to store neonatal blood specimens for at least three years. My objective is to preserve the proteins (both stable and unstable) and nucleic acids (both bacterial and viral RNA and DNA) in blood without compromising their concentrations and conformations. I have gone through a lot of literture regarding blood cryopreservation. They are mostly focused to resuscitation of the cells. I do not care if the cells are live or dead. I just need all the cells, cell surface proteins, soluble proteins and nucleic acids to be in shape. Hope some of you can help me.
Posted 21 April 2010 - 04:12 PM
Posted 21 April 2010 - 08:45 PM
that's what i would love to do. the problem is, I need published reference for the method to pick. can you help me with that?
i have drawn my own blood in citrate tube and zap freezed the tube in liquid nitrogen. i have kept the tubes in -80C. it has been a week.
Posted 22 April 2010 - 04:15 PM
Posted 22 April 2010 - 09:56 PM
I have the protocols for fractionation. Thank you for the help. will keep in touch. will share the updates with you.