Measuring global methylation using real time PCR
Posted 20 April 2010 - 06:40 AM
does anyone have experience measuring global methylation levels using real-time PCR? How sensitive is this method (for LINE-1, ALU etc.)? Who has designed the best assay?
Posted 18 May 2010 - 06:16 PM
However, I am not sure to what extent SNP's for the repeat family members could mask the methylation results you are getting, it is certainly something you should keep in the back of you mind when performing such experiments.
All comments and communication are my own personal ones, and are not tied to any of my affiliations.
Posted 23 September 2010 - 02:23 AM
I agree that SNP's can cause problems. While I was reading some articles about pyrosequencing, I noticed that people more frequently use pyrosequencing, when analyzing methylation status of repetitive elements, than qMSP. I dont fully understand reasons for this.